Screening of native fungal strains from Yaqui Valley, producers of lignolytic enzymes

Sara Roxana Maldonado Bustamante, Iram Mondaca Fernández, Raúl Benjamín Reyes Caro, Luis Angel Gámez Gutiérrez, Sergio de los Santos Villalobos, María Mercedes Meza Montenegro, José de Jesús Balderas Cortés


Introduction: Lignocellulose consists of three polymers: cellulose, hemicellulose and lignin. Biopulping is a pretreatment for lignocellulose using fungi as a means for delignifying it previously to pulping, since fungi may content an enzyme complex including lignin peroxidase, laccase, manganese peroxidase.  Native fungi may have the ability to delignify lignocellulosic material such as wheat straw, wood and others.

Method: This work used ten strains from COLMENA collection at the Microbial Resource Biotechnology Laboratory (Instituto Tecnologico de Sonora). The selection of strains was performed by measuring the halo of enzyme activity of LiP, Lac, Xil during 14 days of incubation and determination of enzymatic index relating the area of enzymatic activity halo against the fungi growth halo, using the software Image J 1.44 for the calculation of the enzymatic potential index.

Results: Ten strains were evaluated and the best strain for LiP was TSM35 with potential index of 3.83±0.29, for Lac and Xil the strain was TSO46 with potential index of 20.37±2.70 and 1.38±0.00 respectively.  With this study it is possible determine the potential for incorporate native fungi in a pretreatment process for delignification (biopulping).

Discussion or Conclusion: From ten strains studied, three strains had ligninolytic activity by excretion lignin peroxidase, lacase and xylanase and they have the potential to be used in pretreatments in a delignification process.


lignin peroxidase; lacase; xylanase; enzimatic potential index


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