Abstract
Introduction: Due to the development of resistance of microorganisms to multiple drugs, exhaustive investigations of therapeutic agents derived from natural sources, such as curcumin, have been carried out. Curcumin (diferuloylmethane) is a phenolic compound, the main curcuminoid present in the species Curcuma longa L. By virtue of its multiple pharmacological properties (antibacterial, antifungal and anti-inflammatory) it has been of great interest to scientists in recent years. The objective of this study was to evaluate the antimicrobial effect of curcumin through the minimum inhibitory concentration (MIC) on strains of Enterococcus faecalis, Escherichia coli, Staphylococcus aureus and Candida albicans.
Method: The in vitro antibacterial activity of curcumin against E. faecalis (ATCC 51299), E. coli (ATCC 25922) and S. aureus (ATCC 25923) was evaluated, following the protocol according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI) in the document M-07-A8. The in vitro antifungal activity of curcumin against C. albicans (ATCC 26790) was evaluated following the protocol according to the CLSI recommendations in the documents M27-A.
Results: The MIC values of curcumin were 125 μg/ml, 31.2 μg/ml, 31.2 μg/ml and 125 μg/ml for E. faecalis, E. coli, S. aureus and C. albicans, respectively.
Conclusion: The results of this study reveal that curcumin in low concentrations has an antibacterial and antifungal effect on strains of E. faecalis, E. coli, S. aureus and C. albicans.
References
Aggarwal, B. B., & Harikumar, K. B. (2009). Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune, and neoplastic diseases. The international journal of biochemistry & cell biology, 41(1), 40-59. DOI: https://doi.org/10.1016/j.biocel.2008.06.010
Aggarwal, B. B., Sundaram, C., Malani, N., & Ichikawa, H. (2007). Curcumin: the Indian solid gold. Advances in experimental medicine and biology, 595, 1-75. DOI: https://doi.org/10.1007/978-0-387-46401-5_1
Aggarwal, B. B., & Sung, B. (2009). Pharmacological basis for the role of curcumin in chronic diseases: an age-old spice with modern targets. Trends in pharmacological sciences, 30(2), 85-94. DOI: https://doi.org/10.1016/j.tips.2008.11.002
Behera, R. & Chandi C. R. (2011). Evaluation of Antibacterial Activity of Turmeric (Curcuma longa L.) Leaf Essential Oils of Three Different States of India against Shigella spp. Journal of Biologically Active Products from Nature, 1(2), 125-131. DOI: 10.1080/22311866.2011.10719079
Cantón, E., Martín E. & Espinel, A. (2001) Pruebas estandarizadas para el estudio de la sensibilidad a los antifúngicos. Revista Iberoamericana de Micología, 15.1-15.7.
Clinical and Laboratory Standards Institute. (2009). Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically. Approved standard. 8th ed. CLSI document M07-A8. Wayne, Penn., USA.
Dovigo, L. N., Pavarina, A. C., Carmello, J. C., Machado, A. L., Brunetti, I. L., & Bagnato, V. S. (2011). Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin. Lasers in surgery and medicine, 43(9), 927-934. DOI: https://doi.org/10.1002/lsm.21110
Gunes, H., Gulen, D., Mutlu, R., Gumus, A., Tas, T., & Topkaya, A. E. (2016). Antibacterial effects of curcumin: An in vitro minimum inhibitory concentration study. Toxicology and industrial health, 32(2), 246-250. DOI: https://doi.org/10.1177/0748233713498458
González, A.J., Sanz, D., Claramunt, R.M., Lavandera, J.L., Alkorta, I. & Elguero J. (2015) Curcumin and curcuminoids: chemistry, structural studies, and biological properties. Anales de la Real Academia Nacional de Farmacia, 81(4), 278-310.
Gupta, S. C., Patchva, S., & Aggarwal, B. B. (2013). Therapeutic roles of curcumin: lessons learned from clinical trials. The AAPS journal, 15(1), 195-218. DOI: https://doi.org/10.1208/s12248-012-9432-8
Hewlings, S. J., & Kalman, D. S. (2017). Curcumin: A Review of Its Effects on Human Health. Foods (Basel, Switzerland), 6(10), 92. DOI: https://doi.org/10.3390/foods6100092
Kali, A., Bhuvaneshwar, D., Charles, P. M., & Seetha, K. S. (2016). Antibacterial synergy of curcumin with antibiotics against biofilm producing clinical bacterial isolates. Journal of basic and clinical pharmacy, 7(3), 93-96. DOI: https://doi.org/10.4103/0976-0105.183265
Khan, N., Shreaz, S., Bhatia, R., Muralidhar S., Nikhat M., & Khan L.A. (2011). Curcumin as a promising anticandidal of clinical interest. Canadian Journal of Microbiology, 57(3), 204-10.
Khan, N., Shreaz, S., Bhatia, R., Ahmad, S.I., Muralidhar, S., Manzoor, N. & Khan, L.A. (2012). Anticandidal activity of curcumin and methyl cinnamaldehyde. Fitoterapia, 83(3), 434-40.
Martins, C. V., da Silva, D. L., Neres, A. T., Magalhães, T. F., Watanabe, G. A., Modolo, L. V., Sabino, A. A., de Fátima, A., & de Resende, M. A. (2009). Curcumin as a promising antifungal of clinical interest. The Journal of antimicrobial chemotherapy, 63(2), 337-339. DOI: https://doi.org/10.1093/jac/dkn488
Moghadamtousi, S. Z., Kadir, H. A., Hassandarvish, P., Tajik, H., Abubakar, S., & Zandi, K. (2014). A review on antibacterial, antiviral, and antifungal activity of curcumin. BioMed research international, 2014, 186864. DOI: https://doi.org/10.1155/2014/186864
Mun, S.H., Joung, D.K., Kim, Y.S., Kang, O.H., Kim, S.B., Seo, Y.S., Kim Y.C., Lee, D.S., Shin, D.W., Kweon, K.T. & Kwon, D.Y. (2013). Synergistic antibacterial effect of curcumin against methicillin-resistant Staphylococcus aureus. Phytomedicine, 20(8–9), 714–8.
Neelofar K., Sheikh S., Bhatia R., Muralidhar S., Manzoor N. & Khan L.A. (2011). Curcumin as a promising anticandidal of clinical interest. Canadian Journal of Microbiology, 57(3), 204-10. DOI: https://doi.org/10.1139/W10-117
Neelofar, K., Sheikh S., Bhatia, R., Ahmad, S.I., Muralidhar, S., Manzoor, N. & Khan, L. A. (2012). Anticandidal activity of curcumin and methyl cinnamaldehyde. Fitoterapia, 83(3), 434-40.
Paul, S., Mohanram, K., & Kannan, I. (2018). Antifungal activity of curcumin-silver nanoparticles against fluconazole-resistant clinical isolates of Candida species. Ayu, 39(3), 182-186. DOI: https://doi.org/10.4103/ayu.AYU_24_18
Shehzad, A., Rehman, G. & Lee, Y. S. (2013). Curcumin in inflammatory diseases. Biofactors, 39(1), 69-77.
Tajbakhsh, S., Mohammadi, K., Deilami, I., Zandi, K., Ramedani, E. & Asayesh, G. (2008). Antibacterial activity of indium curcumin and indium diacetylcurcumin. African Journal of Biotechnology, 7(21), 3832-5.
Tsao, S.M. & Yin, M.C. (2000). Enhanced Inhibitory Effect from Interaction of Curcumin with Amphotericin B or Fluconazole against Candida Species. Journal of Food and Drug Analysis, 8(3), 208-12. DOI: https://doi.org/10.38212/2224-6614.2831
Tyagi, P., Singh, M., Kumari, H., Kumari, A. & Mukhopadhyay, K. (2015). Bactericidal Activity of Curcumin I Is Associated with Damaging of Bacterial Membrane. PLoS ONE, 10(3), e0121313.
Wang, Y., Lu, Z., Wu, H. & Lv, F. (2009). Study on the antibiotic activity of microcapsule curcumin against foodborne pathogens. International Journal of Food Microbiology, 136(1), 71-4. DOI: 10.1016/j.ijfoodmicro.2009.09.001
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