Insights into the mechanisms of Cd hyperaccumulation in S. kali, a desert plant species
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Salsola kali
mecanismos Salsola kali

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de la Rosa, G., Martínez, A., Castillo, H., Fuentes-Ramírez, R., & Gardea-Torresdey, J. (2014). Insights into the mechanisms of Cd hyperaccumulation in S. kali, a desert plant species. Nova Scientia, 1(2), 33–55.


Tumbleweed (S. kali), a desert plant worldwide distributed, has been proposed as a potential Cdhyperaccumulator. X-ray studies showed that thiol and oxygen related compounds are involved in Cd sequestration within the plant. Thus, we have proposed that organic acids, cell wall, phytochelatins, and other glutathione related compounds might be involved in the mechanisms of Cd hyperaccumulation in tumbleweed. In this study, native plants were used to determine Cd content in phloem/xylem tissues and the related biochemical mechanisms of Cd uptake at the protein level. In addition, plant extracts were analyzed by high pressure liquid chromatography (HPLC) to identify and quantify organic acids. Plants were treated with 0, 20, 200, and 400 mg Cd L-1 for 48 h in hydroponic media. Cd incorporation was measured in roots, phloem/cortex, and xylem/pith, separately. It was found that in plants treated with Cd concentrations above 200 mg Cd L-1 , Cd content was higher in phloem than that in xylem. The protein profile in SDSPAGE showed that in Cd-treated plants, two peptides were enhanced while a new peptide was expressed. After G25 gel filtration and Cd codetermination it was found that two proteins (of 29 and 14 kDa) are probably associated to Cd. The use of degenerated primers of the Brassica family allowed the identification of a possible phytochelatin synthase gene. Citric and oxalic were the main acids identified in plant extracts. No significant differences were found in the concentration of citric acid in control and Cd-treated plants. On the other hand, less oxalic acid was quantified in Cd-treated plants as compared to controls. These data indicate that cadmium may have precipitated as oxalate crystals. The results reported herein will be helpful to better understand the mechanisms of Cd hyperaccumulation in S. kali.
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Aldrich, M.V., Gardea-Torresdey, J.L., Peralta-Videa, J.R., Parsons, J.G. 2003. Uptake and Reduction of Cr(VI) to Cr(III) by Mesquite (Prosopis spp.): Chromate-Plant Interaction in Hydroponics and Solid Media Studied Using XAS. Environmental Science and Technology, 37, 1859-1864.

Ausubel Fred M., Brent Roger, Kingston Robert E., Moore David D. 2003. Seidman J.G., Smith John A., Struhl Kevin. Current Protocols in Molecular Biology. John Wiley & Sons. (2003).

Baker, A.J.M., Whiting, S.N., In search of the Holy Grail –a further step in understanding metal hyperaccumulation?. New Phytologist (2002) 155, 1-7.

Bannur, S.V., Kulgod, S.V., Metkar, S.S., Mahajan, S.K., Sainis, J.K. 1999. Protein determination by Ponceau S using digital color image analysis of protein spots on nitrocellulose membranes, Analytical Biochemistry 267, 382-389.

Barceló, J., Poschenrieder, C., Phytoremediation: principles and perspectives. 2003. Contributions to Science, 2(3) 333-344.

Brooks, R.R, Phytochemistry of Hyperaccumulators, In: Plants that hyperaccumulate heavy metals, R.R. Brooks, eds, CAB International, Wallingford, UK, pp 16 (1998).

Cawthray, G.R. 2003. An improved reversed-phase liquid chromatographic method for the analysis of low molecular mass organic acids in plant root exudates. Journal of Chromatography A 1011, 233-240.

Coligan John E., Dunn Hidde Ben M., Ploegh L., Speicher David W., Wingfield Paul T. Current Protocols in Protein Science. John Wiley Current Protocols. (2001)

De la Rosa, G., Martínez-Martínez, A., Pelayo, H., Peralta-Videa, J.R., Sánchez-Salcido, B., Gardea-Torresdey, J. 2005. Production of low molecular weight thiols as a response to cadmium uptake by tumbleweed (Salsola kali), Plant Physiology and Biochemistry, 43, 491-498.

De la Rosa, G., Peralta-Videa, JR., Montes, M., Parsons, JG., Cano-Aguilera, I., Gardea-Torresdey, J.L. 2004. Cadmium uptake and translocation in tumbleweed (Salsola kali), a potential Cd-hyperaccumulator desert plant species: ICP/OES and XAS studies, Chemosphere 55, 1159-1168.

Devlin, R.M, Withman, FH, Plant Physiology, 4th Ed., Wadsworth Publishing Company, Belmont, CA, pp: 114-116 (1983).

Fomina, M., Hillier, S., Charnock, J.M., Melville, K., Alexander, I.J., Gadd, G.M. 2005. Role of Oxalic Acid Overexcretion in Transformations of Toxic Metal Minerals by Beauveria caledonica. Applied and Environmental Microbiology 71, 371-381.

Franceschi, V.R., Horner, H.T. 1980. Calcium oxalate crystals in plants. Botanical Reviews 46, 361-427.

Gong, J.M., Lee, D.A., Schroeder, J.I. 2003. Long-distance root-to-shoot transport of phytochelatins and cadmium in Arabidopsis, Proceedings of the National Academy of Sciences, 100, 10118-10123

Kingston H.M, Jassie L.B (eds) (1988) ACS Professional Reference Book Series, Amer. Chem. Soc., Washington, DC

Lutts, S., Lefèvre, I., Delpérée, C., Kivits, S., Dechamps, C., Robledo, A., Correal, E. 2004. Heavy Metal Accumulation by the Halophyte Species Mediterranean Saltbush. Journal of Environmental Quality, 33, 1271-1279.

Ma, J.F, Nomoto, K. 1996. Effective regulation of iron acquisition in graminaceous plants-the role of mugineic acid as phytosiderophores. Physiologia Plantarum, 97 609-617.

Ma, J.F., Ryan, P.R., Delhaize, E. 2001. Aluminum tolerance in plants and the complexing role of organic acids. Trends in Plant Science, 6, 273-278.

Mejare, M., Bulow, L. 2001. Metal binding proteins and peptides in bioremediation and phytoremediation of heavy metals. Trends in Biotechnology 19, 67-73.

Nakata, P.A. 2003. Advances in our understanding of calcium oxalate crystal formation and function in plants. Plant Science 164, 901-909.

Noret, N., Meerts, P., Tolrà, R., Poschendierer, C., Barceló, J., Escarre, J. 2005. Palatability of Thlaspi caerulescens for snails: influence of zinc and glucosinolates, New Phytologist, 165, 763-772.

Peralta, J.R., Gardea-Torresdey, J.L., Tiemman, K.J., Gomez, E., Arteaga, S., Rascon, E., Parsons, J. 2001. Uptake and effect of five heavy metals on seed germination and plant growth in alfalfa (Medicago sativa). Bulletin of Environmental Contamination and Toxicology. 66, 727-734.

Prasad, M.N.V. Phytoremediation of metals and radionuclides in the environment: the case for natural hyperaccumulators, metal transporters, soil-amending chelators and transgenic plants, In: Prasad, MNV ed., Heavy metal stress in plants, from biomolecules to ecosystems, 2nd Ed., Springer Heidelberg, India (2004), pp: 345-357.

Roosens, N., Verbruggen, N., Meerts, P., Ximenez-Embun, P., Smith, J.A.C. 2003. Natural variation in cadmium tolerance and its relationships to metal hyperaccumulation for seven populations of Thlaspi caerulescens from western Europe, Plant, Cell & Environment 26, 1657-1672.

Schillinger, W.F., Young, F.L. 2000. Soil water use and growth of Russian thistle after wheat harvest, Agronomy Journal, 92, 167-172.

Verret, F., Gravot, A., Auroy, P., Leonhardt, N., David, P., Nussaume, L., Vavasseur, A., Richaud, P. 2004. Overexpression of AtHMA4 enhances root-to-shoot translocation of zinc and cadmium and plant metal tolerance, FEBS Letters 576, 306-312.

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