DNA fingerprint of strawberry varieties developed at Colegio de Postgraduados, Mexico

Martín Aguilar Tlatelpa, Guillermo Calderón Zavala, María Alejandra Gutiérrez Espinosa, Ricardo Lobato Ortiz, Leobigildo Córdoba Téllez, Victor Volke Haller, Marja Liza Fajardo Franco, Amalio Santacruz Varela

Abstract


In a breeding program for strawberry (Fragaria × ananassa) it is important to have a methodology to evaluate the genetic integrity of the plant in all the stages of increase, from different criteria, such as morphological, physiological and molecular; for this purpose one of the most appropriate tools are the Simple Sequence Repeats (SSR`s) molecular markers, since they allow, for example, identifying populations with a reduced genetic diversity, revealing genealogies, knowing the degree of relatedness between individuals, providing solid elements in the defense of intellectual property, evaluation of the purity of the plant material, identification of somaclonal variation and to avoid the mixture of plant material in germplasm banks. In this sense the objective of this study was to obtain the genetic fingerprint of strawberry varieties CP0201, CP0204, CP0615, CPLE7 developed at Colegio de Postgraduados and variety Festival developed at Florida, US, with the use of nine microsatellite (SSR`s) loci. The process included the DNA extraction from strawberry leaf tissue, as well as the amplification by means of PCR of the SSR`s loci grouped in multiplex reactions. The PCR products were separated by capillary electrophoresis and their size in base pairs was determined with the GeneMapper® v. 4 software. From the allele frequencies distance matrices were calculated the Jaccard and Dice coefficients. 63 different alleles were found, each pair of primers amplified between 3 and 12 alleles. The markers that presented the highest number of alleles were EMFn181 (11 alleles) and EMFv104 (12 alleles). The genetic fingerprint of each variety was generated. Differences between the CP0615, CPLE7 and Festival were found based on their allelic profiles; CP0204 and CP0201 had a similar genetic fingerprint, since they are related through their female parent; the allelic diversity index within the populations ranged from 3.96 to 5.93. The varieties had a low uniformity index due to the high level of polymorphism of the markers used.

Keywords


Fragaria × ananassa; allelic profile; SSR, molecular markers; genetic diversity

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