Endomycorrhizal diversity in coffee plants (Coffea arabica L.) infected with rust (Hemileia vastatrix)

Sonia Herrera Monroy, Rogelio Castro Brindis, Jesús Pérez Moreno, Eduardo Valdés Velarde


Coffee (Coffea arabica L.) is one of the most important agricultural products in the world, since more than 56 countries are dedicated to its cultivation and it is the main agricultural commodity since the period 1962-1989 and coffee orange rust (Hemileia vastatrix Berkeley & Broome) is the most devastating disease for this crop. In Central America, the losses caused by this pathogen were up to 50% in the 2011-2012 production cycle. Coffee production in the state of Veracruz decreased by 8% since 2011 due to rust infestations, consequently research has been carried out on beneficial microorganisms that provide tolerance to the pathogen, whose obtaining and application does not represent a risk of deterioration of the soil resource. Therefore, the endomycorrhizal meet these requirements, however it is necessary to know the diversity and conditions of association with the agricultural species of interest so that the management is effective. The objective was to characterize endomycorrhic populations present in coffee plants (C. arabica L.) Typica variety with and without signs of rust infection in Ixhuatlán del café, Veracruz. We identified 37 species that correspond to 14 genera of endomycorrhizal, being the most common Acaulospora and Rhizophagus, the latter being the most frequent in plants without signs of rust infection. A higher percentage of root colonization and spore density was also found in plants without signs of infection (7.70% and 661.68 spores / 100 g of soil, respectively) but the Shannon (H) diversity index and the Simpson (D) does not indicate that the endomycorrhizal populations found are diverse. These results show that the plants that interact in symbiosis with mycorrhiza are able to tolerate better the biotic and abiotic stress, besides the prevalence of certain endomycorrhizal genera in coffee plants with particular physiological characteristics gives a guideline to deepen the study of the established differential symbiosis between plants and microorganisms.


Coffea arabica L.; Hemileia vastatrix; biodiversity; community; endomycorrhizal


Aleksandrowicz-Trzcinska M., Szaniawski A., Studnicki M., Bederska-Błaszczyk M., & Urban A. (2017). The effect of silver and copper nanoparticles on the condition of English oak (Quercus robur L.) seedlings in a container nursery experiment. Forests, 8(9), 310. https://doi.org/10.3390/f8090310.

Alves L., Oliveira V. L., Filho N. S., Federal U., & Catarina D. S. (2010). Utilization of rocks and ectomycorrhizal fungi to promote growth of eucalypt. Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil., 1: 676–684.

Andrade, S. A. L., Mazzafera, P., Schiavinato, M. A., & Silveira, A. P. D. (2009). Arbuscular mycorrhizal association in coffee. Journal of Agricultural Science, 147(2), 105–115. https://doi.org/10.1017/S0021859608008344

Camarena-Gutierrez, G. (2012). Interaccion planta-hongos micorrizicos arbusculares. Revista Chapingo, Serie Ciencias Forestales y Del Ambiente, 18(3), 409–421. https://doi.org/10.5154/r.rchscfa.2011.11.093

Gildon, A. y Tinker, P. B. (1983). Interactions of vesicular-arbuscular mycorrhizal infections and heavy metals in plants. New Phytologist.

INEGI. (2009). Prontuario de Información Geográfica Municipal Ihuatlán del Café, Veracruz de Ignacio de la Llave. México: INEGI.

Talhinhas, P., Batista, D., Diniz, I., Vieira, A., Silva, D. N., Loureiro, A., … Silva, M. do C. (2017). The coffee leaf rust pathogen Hemileia vastatrix: one and a half centuries around the tropics. Molecular Plant Pathology, 18(8), 1039–1051. https://doi.org/10.1111/mpp.12512

Arias R., Heredia-Abarca G., Sosa V., Fuentes-Ramírez L. (2011). Diversity and abundance of arbuscular mycorrhizal fungi spores under different coffee production systems and in a tropical montane cloud forest patch in Veracruz, Mexico. Agroforest System. 85 (1): 179-193.

Augé R. M. (2004). Arbuscular mycorrhizae and soil/plant water relations. Canadian Journal of Soil Science. 84: 373–381.

Bolaños-B. M., Rivillas-Osorio A., Suárez-Vásquez S. (2000). Identificación de micorrizas arbusculares en suelos de la zona cafetalera colombiana. Cenicafé. 51(4): 245-262.

Bora M. y Lokhandwala A. (2016). Mycorrhizal Association: A safeguard for plant pathogen in Hashem, Abeer et al. (2016). “The interaction between arbuscular mycorrhizal fungi and endophytic bacteria enhances plant growth of acacia gerrardii under salt stress.” Frontiers in Microbiology 7: 1–15.

Camarena-Gutierrez G. (2012). Interaccion planta-hongos micorrizicos arbusculares. Revista Chapingo, Serie Ciencias Forestales y Del Ambiente, 18(3), 409–421. https://doi.org/10.5154/r.rchscfa.2011.11.093

Cruz S. J. (1989). Estudio de la simbiosis micorrícica vesicular arbuscular en el cultivo de Coffea arabica var. Caturra. Fitopatologia Colombiana 13, 56–64.

Dobo B., Fassil A., Zebene A. (2018). Effect of tree-tnset-coffee based agro-forestry Practices on arbuscular mycorrhizal fungi (AMF) species diversity and spore density. Agroforestry Systems. 92(2): 525–540.

Del Mar Alguacil M., Torrecillas E., Lozano Z., Roldán A. (2011). Evidence of differences between the communities of arbuscular mycorrhizal fungi colonizing galls and roots of Prunus persica infected by the root-knot nematode Meloidogyne incognita. Applied and Environmental Microbiology. 77(24): 8656–8661.

Etchevers J. D. (2010). Interpretación de los análisis químicos de suelo. Centro de Edafología, Colegio de Postgraduados. México.

Elsen A., Gervacio, D., Swennen, R., De Waele, D. (2008). AMF-induced biocontrol against plant parasitic nematodes in Musa sp.: a systemic effect. Mycorrhiza 18:251–256.

FAOSTAT. (octubre, 2016). La producción de café en México: periodo 2002-2012. Recuperado de: http://biblioteca.catie.ac.cr/royadelcafé/descargas/ROYA-MA.pdf.

FIRA. (octubre, 2016). Panorama Agroalimentario: Café 2016. Dirección de Investigación y Evaluación Económica Sectorial. Recuperado de: https://www.gob.mx/cms/uploads/attachment/file/200636/Panorama_Agroalimentario_Caf__2016.pdf

Gildon, A. y Tinker, P. B. (1983). Interactions of vesicular-arbuscular mycorrhizal infections and heavy metals in plants. New Phytologist.

Gonzalez-Chavez M.C. y Maldonado-Mendoza I.E. 2009. Manual básico para el cultivo de las micorrizas arbusculares, obtención de cultivos monospóricos y monoxénicos. Instituto Politencino Nacional. México. 43 p.

Haddad F., Saravia R. M., Mizubuti S. G., Romeiro R. S., Maffia L. A. (2014). Insolation and selection of Hemileia vastatrix antagonist. European Journal Plant Pathol. 139: 763-772.

Imagen Agropecuaria. (23, mayo, 2017). Estiman caída real del 40% en precio del café en México para ciclo 2017-18. Recuperado de http://imagenagropecuaria.com/2017/estiman-caida-real-del-40-en-precio-del-cafe-en-mexico-ciclo-2017-18/.

INEGI. (2015). Información geográfica y edafoclimatica municipal: Ixhuatlán del Café, Veracruz de Ignacio de la Llave. México: INEGI.

Jackson D., Skillman J., Vandermeer J. (2012). Indirect biological control of the coffee leaf rust, Hemielia vastatrix, by the entomogenous fungus Lecanillium lecanii a complex coffe agroecosystem. Biological Control. 61: 89-97.

Klute A. (1986). Methods of soil analysis. Part 1-Physical and mineralogical methods. American Society of Agronomy -Soil Science Society of America. Madison, WI.

Lee C. S, Lee Y. J., Jeun Y. C. (2005). Observations of infection structures on the leaves of cucumber plants pre-treated with arbuscular mycorrhiza Glomus intraradices after challenge inoculation with Colletotrichum orbiculare. Plant Pathol 21:237–243.

Li Ling-fei, Tao Li, Zhi-wei Zhao. (2007). Differences of arbuscular mycorrhizal fungal diversity and community between a cultivated land, an old field, and a never-cultivated field in a hot and arid ecosystem of southwest china. Mycorrhiza. 17: 655–65.

Litelier A. E. (1967). Manual de fertilizantes para Chile. Banco del Estado, Santiago, Chile. 200 p.

Muleta D., Assefa F., Nemomissa S. & Granhall U. (2007). Composition of coffee shade tree species and density of indigenous arbuscular mycorrhizal fungi (AMF) spores in Bonga natural coffee forest, southwestern Ethiopia. Forest Ecology and Management 241, 145–154.

Muñoz I. D., Mendoza C. A., López G. F., Soler A. A., Hernández M. M. (2007). Edafología manual de métodos de análisis de suelo. Universidad Nacional Autónoma de México. 82 p.

Menezes M. S., Silva D. K. A., Queiroz M. A. A., Félix W. P., Yano-Melo A. M. (5, febrero, 2016). Agriculture, ecosystems and environment arbuscular mycorrhizal fungal communities in buffelgrass pasture under intercropping and shading systems in brazilian semiarid conditions. Agriculture, Ecosystems and Environment. 230, 55–67. Recuperado de https://doi.org/10.1016/j.agee.2016.05.024.

Phillips J. M. & Hayman D. S. (1970). Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment to infection. Trans. Brit. Mycol. Soc. 55: 158-161.

Richars L. A. (1962). Diagnóstico y rehabilitación de suelos sódicos. Traducido al español por N. Sánchez. Instituto Nacional de Investigaciones Agrícolas. 20 p.

Riess S. & Sanvito A. (1985). Investigations on vesicular-arbuscular mycorrhizae in different conditions of coffee cultivations in Mexico. Micologia Italiana 14, 57–62.

Riling, M., Aguilar‐Trigueros, Carlos A., Bergmann, J., Verbruggen, E.,Veresoglou, S. D., Lehmann, A. (2015). Plant root an mycorrhizal fungal traits for understanding soil agreggation. New Phytologist. 205: 1385-1388.

Ruíz-Espinoza F. (23 mayo 2011). Agrohomeopatía conocimiento científico para la conservación de la vida. Centro regional Universitario del Anáhuac. Recuperado de http://www.cenacafe.org.mx/assets/drfelipe2.pdf.

SAGARPA (Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación, MX). (2, enero, 2013). Impactos Café. Recuperado de: http://www.sagarpa.gob.mx/agricultura/Documents/Cultivos%20Agroindustriales/Impactos%20Caf%C3%A9.pdf.

Silva H. S., Tozzi J. P., Terrasan C. R., Bettiol W. (2012). Endhopytic mocroorganism from the coffe tissues as plant growth promoters and biocontrol agents of coffe leaf rust. Biological Control. 63: 62-67.

Somers E., Vanderleyden J., Srinivasan M. (2004). Rhizosphere bacterial signalling: a love parade beneath our feet. Crit. Microbiol. 30(4): 205– 240.

Swamy M.K., Akhtar M.S., Sinniah U.R. (2016). Root Exudates and their molecular interactions with rhizospheric microbes. In: Hakeem K., Akhtar M. (eds) Plant, Soil and Microbes. Springer.438 p.

Tavera S. G. (1985). Criterios para la interpretación y aprovechamiento de los reportes de laboratorio para las áreas de asistencia técnica. Publicación 3.

Talbot, M. 2011. The coffee commodity chain in the world-economy: Arrighl's systemic cycles and braudel's layers of analysis. Revista American Sociological Association. 17 (1): 58-88.

Talhinhas P., Batista D., Diniz I., Vieira A., Silva D. N., Loureiro A., … Silva M. do C. (2017). The coffee leaf rust pathogen Hemileia vastatrix: one and a half centuries around the tropics. Molecular Plant Pathology, 18(8), 1039–1051. https://doi.org/10.1111/mpp.12512

Trejo-Aguilar D., Zulueta-Rodríguez R., Lara-Capistrán L. (2008). Manual de prácticas para el estudio de la simbiosis micorrizógena arbuscular. Textos universitarios, Universidad Veracruzana. 134 p.

Trejo-Aguilar D. (2003). Efecto de la endomicorriza arbuscular sobre el desarrollo de nemátodos fitopatógenos en plántulas de café. Tesis doctoral. Universidad Nacional Autónoma de México. 165 p.

Wheeler B. (23, enero, 2017). International Culture Collection of (vesicular) arbuscular mycorrhizal fungi. Recuperado de http://fungi.invam.wvu.edu/the-fungi/species-descriptions.html.

DOI: https://doi.org/10.21640/ns.v11i22.1642


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