Detection of polysaccharides in Ganoderma lucidum extracts

Ma. Dolores Castañeda Antonio, Daniel Claudio Martínez Carrera, José Antonio Rivera Tapia, Roberto Portillo Reyes, Porfirio Morales Almora, Mercedes Sobal Cruz, Antonio Macías López, Hermilo Leal Lara


Introduction: Ganoderma lucidum is one of the most worldwide known medicinal mushrooms. However, this species of mexican mushroom has notable differences compared to its similar one since it has not been studied enough so it is important to determine its characteristics once it has been cultivated and its extracts have obtained in a standardized form.

Method: The polysaccharides of the extracts of Ganoderma lucidum strain CP-145 were measured by the quantum magnetic resonance analyzer MARSIII of Bruce Copen (MARSIII), a proposed technique to estimate values of compounds in the agricultural area, these results were compared with two standardized techniques: Fehling method of sugar reducers and Gas Chromatography - Mass spectrometry (GC-MS), the extracts to be measured were obtained from the traditional culture of basidiocarps (control) and extract of basidiocarps added with acetylsalicylic acid (ASA).

Results: The results for the MARSIII quantum magnetic analyzer were 4.967 ± 1.016 and 8.110 ± 1.416% in control extract and extract added respectively, by the Fehling´s method 8.784 ± 2.019% and 41.326 ± 1.430% in control extract and extract added respectively, for CG- MS 7.050 ± 1.527 and 18.456 ± 2.937% in control extract and extract added respectively.                      

Discussion and Conclusion: Significant differences were found on the Fehling´s techniques and on the GC-MS for both extracts, but not in the MARSIII analyzer detection, which indicates that it is not an adequate method to detect variations in the concentrations of polysaccharides in this type of extracts.



M.A.R.S.III.; chromatography; Fehling´s method; extracts; acetylsalicylic acid

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