Evaluation of DNA vaccine and its recombinant peptide against inhibin alpha (165-300) on immune response and fertility in mice

Carlos Luna-Palomera, Juan González-González, Amanda Gayosso-Vázquez, Vianey Ramírez-Andoney, Nadia Florencia Ojeda-Robertos, Julio Armando Aguilar-Cabrales, Rogelio Alejandro Alonso-Morales


The aim was to evaluate a DNA vaccine (INHA-DNA) and its recombinant peptide INHA-rec) against inhibin alpha (INHA) on the immune response and fertility in mice. Sixteen Balb/c mice were divided into two groups of 8 each and under a heterologous prime boost immunogenic scheme one group were inoculated with 100 μg of INHA-DNA and 14 d later with 100 μg of INHA-rec. The control group received saline solution. After the first birth, a booster was given with both antigens (INHA-DNA + INHA-rec). Immunization with 100 μg of INHA-DNA and INHA-rec induced high levels of anti-INHA antibody, as well as greater weight and size litter (P <0.05) compared to control. The mean fertility was similar between INHA (67%) and control (60%). In conclusion, the DNA vaccine and its recombinant antigen against INHA are capable of inducing immunity against INHA by increasing litter size without an apparent negative effect on fertility in mice.


Inhibin alfa; DNA vaccine; fertility; recombinant peptide


Dan X, Han L, Riaz H, Luo X, Liu X, Chong Z, Yang L. (2016). Construction and evaluation of the novel DNA vaccine harboring the inhibin α (1–32) and the RF-amide related peptide-3 genes for improving fertility in mice. Experimental Animals 65: 17–25.

Dan X, Han L, Riaz H, Luo X, Liu X, Chong Z, Yang L. (2016). Effect of the novel DNA vaccine fusing inhibin α (1-32) and the RF-amide related peptide-3 genes on immune response, hormone levels and fertility in Tan sheep. Anim Reprod Sci 164: 105 – 110.

D'Alessandro A, Martemucci G, Iaffaldano N. (1999). Active immunization with a synthetic fragment of pig inhibin -subunit increases ovulation rate and embryo production in superovulated ewes but season affects its efficiency. J Reprod Fetil 115: 185-191.

de Kretser DM, Hedger MP, Loveland KL, Phillips DJ. (2002). Inhibins, activins and follistatin in reproduction. Hum Reprod 8: 529-541.

Glencross RG, Bleach EC, Wood SC Knight PG. (1994). Active immunization of heifers against inhibin: effects on plasma concentrations of gonadotrophins, steroids and ovarian follicular dynamics during prostaglandin synchronized cycles. J Reprod Fertil 100: 599–605.

Han L, Mao DG, Zhang DK, Liang AX, Fang M, Muhammad Moaeen-ud-Din, Yang LG. (2008). Development and evaluation of a novel DNA vaccine expressing inhibin a (1–32) fragment for improving the fertility in rats and sheep. Anim Reprod Sci 109:251-265.

Han L, Zhen YH, Liang AX, Zhang J, Riaz H, Xiong JJ, Guo AZ, Yang LG. (2014). Oral vaccination with inhibin DNA delivered using attenuated Salmonella choleraesuis for improving reproductive traits in mice. J Basic Microbiol 54: 962-968.

Knight PG. (1996). Roles of inhibins, activins and follistatin in the female reproductive system. Front Neuroendocrin 17:476-509.

Knight PG, Satchell L, Glister C. (2012). Intra-ovarian roles of activins and inhibins. Mol. Cell. Endocrinol. 359 (1–2): 53-65.

King BF, Britt JH, Esbenshade KL, Flowers WL, Sesti LA, Martin TL. (1993). Ovulatory and endocrine responses after active immunization of gilts against a synthetic fragment of bovine inhibin. J Anim Sci 71:975-982.

Kutzler MA Weiner DB. (2008). DNA vaccines: ready for prime time? Nat Rev Genet 9: 776–788.

Lu S. (2009). Heterologous Prime-Boost Vaccination. Current Opinion in Immunology 21: 346–351.

Medan MS, Takedom T, Aoyagi Y, Konishi M, Yazawa S, Watanabe G Taya K. (2006). The effect of active immunization against inhibin on gonadotropin secretions and follicular dynamics during the estrous in cows. J Reprod Dev 52: 107–113.

Medan MS, Watanabe G, Sasaki K, Nagura Y, Sakaime H, Fujita M, Sharawy S Taya K. (2003). Ovarian and hormonal response of female goats to active immunization against inhibin. J Endocrinol 177: 287–294.

Morris DG, McDermott MG, Diskin MG, Morrison CA, Swift PJ Sreenan JM. (1993). Effect of immunization against synthetic peptide sequences of bovine inhibin a-subunit on ovulation rate and twin-calving rate in heifers. J Reprod Fert 97: 255–261.

Niess JH, Brand S, Gu X, Landsman L, Jung S, McCormick BA, Vyas JM, Boes M, Ploegh HL, Fox JG, Littman DR, Reinecker HC. (2005). CX3CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance. Science 307: 254-258.

Padilla G, Knight PG Holtz W. (2007). Superovulation and embryo collection in nulliparous Boer goat does immunized against a recombinant ovine α-subunit inhibin. Small Ruminant Res 74: 159–164.

Rivier J, Spiess J, McClintock R, Vaughan J, Vale W. (1985). Purification and partial characterization of inhibin from porcine follicular fluid. Biochem Bioph Res Comm. 133 (1): 120-127.

Wang SL, Han L, Ahmad S, Cao SX, Xue LQ, Xing ZF, Feng JZ, Liang AX, and Yang LG. (2012). Effect of a DNA vaccine harboring two copies of inhibin α (1-32) fragments on immune response, hormone concentrations and reproductive performance in rats. Theriogenology 78: 393– 401.

DOI: https://doi.org/10.21640/ns.v10i20.1310


  • There are currently no refbacks.

Copyright (c) 2018 Nova Scientia


Nova Scientia is a multidisciplinary, electronic publication that publishes biannually in the months of May and November; it is published by the Universidad De La Salle Bajío and aims to distribute unpublished and original papers from the different scientific disciplines written by national and international researchers and academics. It does not publish reviews, bibliographical revisions, or professional applications.

Nova Scientia, year 10, issue 21, November 2018 – April 2019, is a biannual journal printed by the Universidad De La Salle Bajío, with its address: Av. Universidad 602, Col. Lomas del Campestre, C. P. 37150, León, Gto. México. Phone: (52) 477 214 3900, e-mail: http://nova_scientia.delasalle.edu.mx. Chief editor: Ph.D. Ramiro Rico Martínez. ISSN 2007 - 0705. Copyright for exclusive use No. 04-2008-092518225500/102, Diffusion rights via computer net 04 - 2008 – 121011584800-203 both granted by the Instituto Nacional del Derecho de Autor.

Editor responsible for updating this issue: Direction of Research Department of the Universidad De La Salle Bajío, last updated on November 23th, 2018.