Productivity of Stevia rebaudiana Bertoni with different irrigation slides and microbial inoculants

Rodrigo Cauich Cauich, Alfonso Pérez Gutiérrez, Mónica Guadalupe Lozano Contreras, René Garruña Hernández, Esaú Ruíz Sánchez

Abstract


Introduction: The leaves are the most important organ in the plant of Stevia rebaudiana Bertoni, because in them is the greater amount of sweetener. Leaf emission is determined by adequate root growth and the amount of water available in the soil. The objective of the present study was to evaluate the respond of S. rebaudiana Bert plants to four irrigation sheets calculated from reference evapotranspiration (ETo) in interaction with microbial inoculants.

Method: The treatments consisted of irrigate with 60, 80, 100 and 120% of the estimated ETo with an "A" type evaporimeter tank in previously inoculated plants. A mixture of Bacillus spp. and Azospirillum brasilense, Rhizophagus intraradices and a control (uninoculated). A randomized complete block design with bi-factorial arrangement and four replicates was used. Growth, production and distribution parameters of dry biomass, gas exchange and water efficiency were evaluated.

Results: Statistical difference (P≤0.05) was found by separate factors. The best height was obtained with 120% ETo, although it was statistically equal (P≤0.05) to 100% ETo registering 42.86 and 40.58 cm respectively, 120% ETo was recorded greater leaf area. Treatment with Rhizophagus intraradices showed significant differences (P≤0.05) in the root length against the control, but not against the mixture of rhizobacteria. In the same way R. intraradices improved the root volume by registering 2.49 cm3 with respect to the control, and allowed to reduce the application of ETo by 20% to obtain the highest production of dry leaf and root biomass. The interaction R. intraradices + 80% ETo favored the assimilation of CO2 with 5.41 μmol m-2 s-1, thus providing better conditions for transpiration and efficient water use (1.87 μmol CO2 mmol-1 H20).

Conclusion: The irrigation factor showed effect on leaf height, leaf area, stem diameter and dry leaf yield, while inoculating factor only affected root length, root volume, MSV / MRS ratio and yield. Finally, the interaction of the two factors was only significant in the parameters of dry biomass production and gas exchange.

Keywords


sweet grass; efficient use of water; biofertilizers; transpiration

References


Aguirre, M. J. F., G. M. B. Irizar, P. A. Duran, C. O. A. Grajeda, M. A. Peña del Rio, O. C. Loredo, B. A. Gutiérrez. (2009). Los biofertilizantes microbianos: alternativa para la agricultura en México. INIFAP. Campo Experimental Rosario Izapa, Tuxtla Chico, Chiapas, México. Folleto técnico (5), 78.

Arizaleta, M., y Pire, R. (2008). Respuesta de plántulas de cafeto al tamaño de la bolsa y fertilización con nitrógeno y fosforo en vivero. Agrociencia. 42(1), 47-55.

Balaguera, H. E., Álvarez, H., J. G.., Rodríguez, J. D. (2008). Efecto del déficit de agua en el trasplante de plántulas de tomate (Solanum lycopersicum L.). Agronomía Colombiana 26(2), 246-255.

Brandle, J. E., Starratt, A. N., Gijzen, M. (1998). Stevia rebaudiana: Its agricultural, biological, and chemical properties. Canadian Journal of plant science. 78(4), 527-536.

Casierra-Posada, F. E., Rodríguez, S. Y. (2006). Tolerancia de plantas de feijoa (Acca sellowiana [Berg] Burret) a la salinidad por NaCl. Agronomía colombiana. 24(2), 258-265.

Compant, S., Clément, C., Sessitsch, A. (2010). Plant growth-promoting bacteria in the rhizo- and endosphere of plants: their role, colonization, mechanisms involved and prospect for utilization. Soil Biology & Biochemmistry. 42(5), 669-678.

Doorenbos, J., W. O. Pruitt. (1977). Guidelines for predicting crop water requirements. Irrigation and drainage paper 24. FAO. Rome, Italy. 24, 1-144.

Fabeiro, C., de Santa Olalla, F. M., De Juan, J. A. (2002). Production of muskmelon (Cucumis melo L.) under controlled deficit irrigation in a semi-arid climate. Agricultural Water Management. 54(2), 93-105.

García, E., Villafañe, R., Basso, C., Florentino, A. (2015). Dinámica de la humedad del suelo y su efecto sobre el rendimiento de la Stevia. Venesuelos 23, 5-10.

Garruña-Hernández, R., Orellana, R., Larque-Saavedra, A., Canto, A. (2014). Understanding the physiological responses of a tropical crop (Capsicum chinense Jacq.) at high temperature. PLoS ONE, 9(11), 1-9.

Gil, R. (2007). El ambiente del suelo y el crecimiento de las raíces. INTA, Estación Experimental Agropecuaria Rafaela. Información técnica de trigo y otros cultivos de invierno. Publicación miscelánea. 107, 12-16.

Godoy Avila, C., Pérez Gutiérrez, A., Torres, E., C. A., Hermosillo, L. J., Reyes, J., I. (2003). Uso de agua, producción de forraje y relaciones hídricas en alfalfa con riego por goteo subsuperficial. Agrociencia 37(2), 107-115.

Lavini, A., Riccardi, M., Pulvento, C., De Luca, S., Scamosci, M., d’Andria, R. (2008). Yield, quality and water consumption of Stevia rebaudiana Bertoni, grown under different irrigation regimes in southern Italy. Ital. J. Agron. / Riv. Agron. 3(2), 135-143.

Lozano-Contreras, M. G., Ramírez-Jaramillo, G. (2015). Enraizamiento de esquejes de estevia con biofertilizantes. Centro de investigación regional sureste. Campo experimental Mocochá. Mérida, Yucatán. Desplegable para productores No. 6.

Lubbe, A., Verpoorte, R. (2011). Cultivation of medicinal and aromatic plants for specialty industrial materials. Industrial crops and products. 34 (1), 785-801.

Lugtenberg, B., Kamilova, F. (2009). Plant growth promoting rhizobacteria. Annual Review Microbiology 63, 541-556.

Martínez Cruz, M. (2015). Stevia rebaudiana (Bert.) Bertoni. Una revisión. Cultivos Tropicales. 36(1), 5-15.

Márdero, S., Nickl, E., Schmook, B., Schneider, L., Rogan, J., Christman, Z., Lawrence, D. (2012). Sequías en el sur de la península de Yucatán: análisis de la variabilidad anual y estacional de la precipitación. Investigaciones geográficas, Boletín del Instituto de Geografía-UNAM 78, 19-33.

Medrano, H., Bota, J., Cifre, J., Flexas, J., Ribas-Carbó, M., Gulías, J. (2007). Eficiencia en el uso del agua por las plantas. Investigaciones geográficas (Esp). 43, 63-84.

Mohamed, S. M., El-Ghait, E. M., Youssef, A. S. M., Khalil, A. M. M., Attia, K. E. (2012). Effect of irrigation rate and some fertilization treatments on vegetative growth and chemical composition of Stevia rebaudiana. Annals of Agric. Sci., Moshtohor, 50(4), 435-446.

Navi, V., Shivakumar, B. S., Suresh, C. K., Earanna, N. (2010). Effect of Glomus fasciculatum and plant growth promoting rhizobacteria on growth and yield of Ocimum basilicum. Karnataka Journal of Agricultural Sciences. 19(1), 17-20.

Pérez-Gutiérrez, A., Pineda-Doporto, A., Latournerie-Moreno, L., Pam-Pech, W., Godoy-Ávila, C. (2008). Niveles de evapotranspiración potencial en la producción de chile habanero. Terra Latinoamericana. 26(1), 53-59.

Pire, R., Valenzuela, I. (1995). Estimación del área foliar en Vitis vinifera L. 'French Colombard' a partir de mediciones lineales en las hojas. Agronomía tropical. 45(1), 143-154.

Quintal Ortiz, W. C., Pérez-Gutiérrez, A., Latournerie Moreno, L., May-Lara, C., Ruiz Sánchez, E., Martínez Chacón, A. J. (2012). Water Use, Water Potential and Yield of Habanero Pepper (Capsicum chinense Jacq.). Revista Fitotecnia Mexicana. 35(2), 155-160.

Ramírez, J. G., Avilés, B. W., Moguel, O. Y., Góngora, G. S., May, L. C. (2011). Estevia (Stevia rebaudiana Bertoni) un cultivo con potencial productivo en México. Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias. Centro de Investigación Regional Sureste. Publicación especial. (4), 84.

Sekhar, K. M., Rachapudi, V. S., Mudalkar, S., Reddy, A. R. (2014). Persistent stimulation of photosynthesis in short rotation coppice mulberry under elevated CO2 atmosphere. Journal of Photochemistry and Photobiology B: Biology. 137, 21-30.

Valladares, F., Vilagrosa, A., Peñuelas, J., Ogaya, R., Camarero, J. J., Corcuera, L., Gil-Pelegrín, E. (2004). Estrés hídrico: ecofisiología y escalas de la sequía. Ecología del bosque mediterráneo en un mundo cambiante. 2, 165-192. Ministerio de Medio Ambiente, EGRAF, S.A., Madrid.




DOI: https://doi.org/10.21640/ns.v10i20.1166

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Nova Scientia

Scope

Nova Scientia is a multidisciplinary, electronic publication that publishes biannually in the months of May and November; it is published by the Universidad De La Salle Bajío and aims to distribute unpublished and original papers from the different scientific disciplines written by national and international researchers and academics. It does not publish reviews, bibliographical revisions, or professional applications.

Nova Scientia, year 10, issue 20, May – October 2018, is a biannual journal printed by the Universidad De La Salle Bajío, with its address: Av. Universidad 602, Col. Lomas del Campestre, C. P. 37150, León, Gto. México. Phone: (52) 477 214 3900, e-mail: http://nova_scientia.delasalle.edu.mx. Chief editor: Ph.D. Ramiro Rico Martínez. ISSN 2007 - 0705. Copyright for exclusive use No. 04-2008-092518225500/102, Diffusion rights via computer net 04 - 2008 – 121011584800-203 both granted by the Instituto Nacional del Derecho de Autor.

Editor responsible for updating this issue: Direction of Research Department of the Universidad De La Salle Bajío, last updated on May 25th, 2018.