Ob Gene Polymorphisms in Holstein Cattle at Comarca Lagunera, Mexico

Sarai Shesareli Mendoza-Retana, Miguel Ángel Gallegos-Robles, Uriel González-Salas, José Luis García-Hernández, Manuel Fortis-Hernández, Cirilo Vázquez-Vázquez, Hector Idilio Trejo-Escareño


The Laguna Region is the most important dairy region of Mexico. Currently are using various techniques to genetically evaluate the animal at an early age, allowing to select future breeding with desirable characteristics. Among related genes to milk production, are the Ob gene also called Leptin gene which acts on the central nervous system and peripheral tissues playing an important role in regulating modulation of appetite, body weight gain, increased metabolism energy and muscle anabolism. This study was performed to determine polymorphism restriction fragment length ACI I of Leptin gene in exon 2 and correlate it with the production parameters and milk quality. Blood sample of one hundred cows were collected on the dairy farm "Lacteos Florida" Francisco I. Madero municipality of Coahuila, Mexico with three status of milk production: high, medium and low. DNA extraction was performed by the modified method of Salting-Out. PCR of leptin gene was performed resulting in a fragment of 272 bp in length and was subjected to PCR-RFLP with the ACI I restriction enzyme and sequencing, correlating TT, CT and CC genotypes with groups of cows sampled high, medium, low producers respectively. Statistical analysis indicated that cows carrier the homozygous genotype (TT) have a significant effect (P <0.01) with respect to the characteristics of production and quality of milk since they had a higher feed intake, weight gain, and a high milk production compared to heterozygote (CT) and homozygote (CC) genotypes. The results show that molecular identification of Ob gene polymorphisms can be used as a tool for genetic selection in Holstein cattle.


leptin; genotypes; quality; milk production


Ayres, D. R., Souza, F. R. P., Mercadante, M. E. Z, Fonseca, L. F. S., Tonhati, H., Cyrillo, J. N. S. G., Bonilha, S. F. M., Albuquerque, L. G. (2010). Evaluation of TFAM and FABP4 gene polymorphisms in three lines of Nellore cattle selected for growth. Journal of Genetics and Molecular Research. 9(4): 2050-2059.

Banos, G., Woolliams, J. A., Woodward, B. W., Forbes, A. B., Coffey, M. P. (2007). Impact of Single Nucleotide Polymorphisms in Leptin, Leptin Receptor, Growth Hormone Receptor, and Diacylglycerol Acyltransferase (DGAT1) Gene Loci on Milk Production, Feed, and Body Energy Traits of UK Dairy Cows. American Dairy Science Association. 91: 3190–3200.

Buchanan, F.C., Fitzsimmons, C.J., Van Kessel, A.G., Thue, T.D., Windkelman-Sim, D.C., & Schmutz, S.M. (2002). Association of a missense mutation in the bovine leptin gene with carcass fat content and leptin mRNA levels. Genet. Sci. Evol. 34: 105-116

Casas, E., White, S. N., Shackelford, S. D., Wheeler, T. L., Koohmaraie, M., Bennett, G. L., Smith, T. P. L. (2007). Assessing the association of single nucleotide polymorphisms at the thyroglobulin gene with carcass traits in beef cattle. American Society of Animal Science. 85: 2807-2814.

Cerón-Muñoz. M. F., Montoya, A, A. E., Trujillo, B. E. R., Ramírez, T, E. J., Monsalve, F. Z. I. (2009). Marcadores del Gen Leptina en Bovinos Cruzados con Angus, Cebú, Romosinuano y Blanco Orejinegro. Revista Científica. FCV-LUZ. 19(4): 371-381.

Choudhary, V., Kumar, P., Bhattacharya, T. K., Bhushan, B., Sharma, A. (2005). DNA polymorphism of leptin gene in Bos indicus and Bos taurus cattle. Genetics and Molecular Biology. 28(4): 740-742.

Corva, P. M., Fernández, M. G., Motter, M., Soria, L., Villarreal, E. L., Schor, A., Mezzadra, C., Melucci, L. M., Miquel, M. C. (2007). Efecto de polimorfismos en el gen de leptina sobre aptitudes carniceras de novillos correspondientes a biotipos europeos y cebuinos. Revista Argentina de Producción Animal. 27(1): 239-248.

Dias, S. A. K., Bermal, C. R., Giachetto, P. F. (2007). Gene da Leptina em Ruminantes. Revista electrónica de Veterinaria. 8(12):1-13.

Frühbeck, Gema. (2006). Intracellular signaling pathways activated by leptin. Biochem Journal. 393: 7-20.

Huang, H., Wei, Y., Meng, Z., Zhang, Y., Liu, X., Guo, L., Lou, J., Chen, G., Lin, H. (2014). Polymorphisms of leptin-b gene associated with growrh traits in orange.-spotted grouper (Epinephelus coioides). International Journal of Molecular Sciences. 15: 11996-12006.

Javanmard, A., Khaledi, K., Asadzadeh, N., Solimanifarjam, A. R. (2010). Detection of polymorphisms in the bovine leptin (LEP) Gene: Association of single nucleotide polymorphism with breeding value of milk traits in Iranian Holstein cattle. Journal of Molecular Genetics 2:10-14.

Liefers, S. C., Pas, M. F., Veerkamp, R. F., Van der, L. T. (2002). Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in Holstein heifers. Journal of Dairy Science. 85(6): 1633–1638.

Martínez, N. C. A., Manrique, P. C., Elzo, M. A. (2012). Cattle genetic evaluation: a historical perception. Revista Colombiana Ciencias Pecuarias. 25: 293-311.

Mojtaba, R., Ziaeddin, M. S., Hossein, Z. N. G. (2015). Análisis de PCR-RFLP del gen de leptina y su asociación con características de la leche en ganado nativo (Bos indicus) de Irán. Revista Mexicana de Ciencias Pecuarias; 6(1): 15-24.

Montoya, A. A. E., Cerón, M. M. F., Trujillo, B. E., Ramirez, T. E. J., Angel, M. P. A. (2009). Frecuencia de los marcadores del gen leptina en razas bovinas criollas y colombianas: I. Romosinuano, Chino Santandereano, Sanmartinero y Velásquez. Revista Científica FCV-LUZ. 19(1): 38 – 48.

Ngu, N. T., Quynh, L. T., Hon, N. V., Nhan, N. V., Khoa, D. V., Hung, L. T., Xuan, N. H. (2015). Influence of Leptin genotypes on milk fat and protein content of crossbred Holstein Friesian x lai sind cows. The Journal of Animal and Plant Sciences. 25: 304-308

Nkrumah, J.D., LI, C., YU, J., Hansen, C., Keisler, D. H., Moore, S. S. (2005). Polymorphisms in the bovine leptin promoter associated with serum leptin concentration, growth, feed intake, feeding behavior, and measures of carcass merit. Journal of Animal. Science. 83(1): 20-28.

Piñeira, J., Río, J., Floody, H., Felmer, R. (2012). Distribución de polimorfismos asociados al grado de infiltración de grasa intramuscular en siete razas bovinas de carne utilizadas en la Región de La Araucanía, Chile. Revista de Medicina Veterinaria 44: 43-52.

Quintero, V. J. C., Ruíz, C. Z. T. (2008). Efectos de la leptina en el inicio de la pubertad en animales machos. Revista Colombiana de Ciencias Pecuarias. 21: 97-108.

Raoof, A. A., Eftekhari, S. F., Reza N. M., Heravi, M. A., Heydarpour, M., Sadeghi, B. (2008). Association of SNP in the exon II of leptin gene with and reproducction traits in holstein iranian Cows. Biotechnology. 7(2): 347-350.

Rivera, A. M., Rojas, E. M., Zapata, D. P. (2010). Protocolo de extracción de DNA por salting-out para pequeños volúmenes de sangre. Revista de Ciencia y Tecnología. 14:4-7.

Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (SAGARPA). 2015. Anuario estadístico de la producción agropecuaria. Delegación Comarca Lagunera https://www.gob.mx/siap/documentos/poblacion-ganadera. (11 Julio, 2015).

Schenkle, F. S., Miller, S. P., Ye, X., Moore, S. S., Nkrumah, J. D., Li, C., Yu, J., Mandell, I. B., Wilton, J. W.,Williams, J. L. (2005). Association of single nucleotide polymorphism in the leptin gene with carcass and meat quality traits of beef cattle. Journal of Animal Science. 83: 2009-2020.

Souza, F. R. P., Mercadante, M. E. Z., Fonseca, L. F. S., Ferreira, L. M. S. Regatieri, I. C., Ayres, D. R., Tonhati, H., Silva, S. L., Razook, A. G., Albuquerque, L. G. (2010). Assessment of DGAT1 and LEP gene polymorphisms in three Nelore (Bos indicus) lines selected for growth and their relationship with growth and carcass traits. American Society of Animal Science. 88: 435-441.

Thomas, M. G., Enns, R. M., Hallford, D. M., Keisler, D. H., Obeidat, B. S., Morrison, C. D., Hernandez, J. A., Bryant, W. D., Flores, R., Lopez, R., Narro, L. (2002). Relationships of metabolic hormones and serum glucose to growth and reproductive development in performance tested Angus, Brangus, and Brahman bulls. Journal of Animal Science. 80(3): 757–767.

Vallone, R., Camiletti, E., Exner, M., Mancuso, W., Marini, P. (2014). Análisis productivo y reproductivo de vacas lecheras Holstein, Pardo Suizo y sus cruzas en un sistema a pastoreo. Revista Veterinaria. 25(1): 40-44.

Vásquez, R. E., Ballesteros, H. H., Muñoz, C. A. (2007). Factores asociados con la calidad de la carne. I parte: la terneza de la carne bovina en 40 empresas ganaderas de la región Caribe y el Magdalena Medio. Revista Corpoica – Ciencia y Tecnología Agropecuaria. 8(2): 60-65.

White, S. N., Casas, E., Wheeler, T. L., Shackelford, S. D., Koohmaraie, M., Riley, D. G., Chase, C. C., Johnson, D. D., Keele, J. W., Smith, T. P. L. (2005). A new single nucleotide polymorphism in CAPN1 extends the current tenderness marker test to include cattle of Bos indicus, Bos taurus, and crossbred descent. Journal of Animal Science. 83(9): 2001–2008.

Woronuk, G. N., Marquess, F. L., James, S. T., Palmer, J., Berryere, T., Deobald, H., Howie, S., Kononoff, P. J. (2011). Association of leptin genotypes with beef cattle characteristics. Journal of Animal Genetics. 68:1-3.

Yoon, D. H., Cho, B. H., Park, B. L., Choi, Y. H., Cheong, H. S., Lee, H. K., Chung, E. R., Cheong, I. C., Shin, H. D. (2005). Asian-Australasian Journal of Animal Sciences. 18(11): 1548-1551.

DOI: https://doi.org/10.21640/ns.v9i19.1028


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